mouse anti complex iii subunit core 1 oxphos  (Thermo Fisher)

Journal: PLoS ONE

Article Title: Deregulation of Mitochondria-Shaping Proteins Opa-1 and Drp-1 in Manganese-Induced Apoptosis

doi: 10.1371/journal.pone.0091848

Figure Lengend Snippet: Opa-1 and Drp-1 expression levels. Monoclonal antibodies that recognize Opa-1 or Drp-1 were used for immunoblotting analysis of total cell lysates. Reprobing with an anti-β-Actin antibody was performed to normalize for protein loading. Signals were quantified with the Image J software. Images correspond to one representative experiment (n = 3). Results are expressed as a percentage of the respective control considered as 100%. Asterisk [*] denotes Opa-1 cleavage product with a MW ∼71 kDa ( A ); Enriched-mitochondrial and cytosolic fractions were subjected to immunoblotting procedure using antibodies that recognize Opa-1 or Drp-1 proteins. Reprobing with anti-Complex III subunit core 1- OxPhos (Complex III) and anti-β-Actin antibodies was performed to normalize for loading control. Images correspond to one representative experiment (n = 2). Results are expressed as a percentage of the respective control considered as 100% ( B ). Statistically significant differences between the controls and experimental groups are indicated by: *p<0.05, **p<0.01 and ***p<0.001 vs. control. AU: Arbitrary Units.

Article Snippet: Mouse anti- complex III subunit core 1-OxPhos (459140) (1∶5000) was obtained from Molecular Probes (Invitrogen, Eugene, OR, USA) and mouse anti- Opa-1 (612607) (1∶1000) and mouse anti- Drp-1 (611113) (1∶400) were from BD Pharmingen (San Diego, CA, USA).

Techniques: Expressing, Western Blot, Software

Journal: PLoS ONE

Article Title: Deregulation of Mitochondria-Shaping Proteins Opa-1 and Drp-1 in Manganese-Induced Apoptosis

doi: 10.1371/journal.pone.0091848

Figure Lengend Snippet: Opa-1 expression levels in total cell lysates. Anti-β- Actin antibody was used as a loading control ( A ); Drp-1 expression levels in enriched-mitochondrial and cytosolic fractions. Membranes were stripped and reprobed for anti-Complex III subunit core 1- OxPhos (Complex III) I and anti-β- Actin as loading control ( B ); CsA diminishes Mn-induced mitochondrial fragmentation, loss of Δψm and apoptotic nuclei appeareance. Quantification of mitochondria ( C ) and nuclei morphology ( D ) classified according to Figs. 4, 5. Scale bar: 10 µm. Three independent experiments were conducted. Statistical significance, **p<0.01 and ***p<0.001 vs. control; ##p<0.01 vs. Mn. Arrowheads: apoptotic (condensed and fragmented chromatin) nuclei.

Article Snippet: Mouse anti- complex III subunit core 1-OxPhos (459140) (1∶5000) was obtained from Molecular Probes (Invitrogen, Eugene, OR, USA) and mouse anti- Opa-1 (612607) (1∶1000) and mouse anti- Drp-1 (611113) (1∶400) were from BD Pharmingen (San Diego, CA, USA).

Techniques: Expressing